Agilent Technologies introduces new application for quantitative end-point RT-PCR gene expression measurement

5/14/2002 - Agilent Technologies Inc. (NYSE: A) today announced a new application for the measurement of RT-PCR using the Agilent 2100 bioanalyzer and standardized PCR from Gene Express National Enterprises Inc. The Agilent 2100 bioanalyzer can be used in combination with Standardized RT (StaRT)-PCR reagents from Gene Express National Enterprises (G.E.N.E.) Inc. (Huntsville, Ala., U.S.) to obtain highly reproducible, standardized gene expression measurement with RT-PCR. The internal standards used in StaRT-PCR control for variable loading of PCR products into electrophoresis channel, and allow quantitative gene expression measurement at the endpoint of PCR, without real-time analysis. Gene expression measurement of 15 genes was more reproducible with the Agilent 2100 bioanalyzer than with the ABI Prism 310 Genetic Analyzer or agarose gel electrophoresis methods.

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